Rhizoponics, the story behind the paper

About two years ago, we (Laura Mathieu, Pierre Tocquin, Claire Périlleux and myself) started to think about an efficient way to monitor the development of the Arabidopsis root system from the seedling to the flowering stages. Why we needed something new... The first obvious solution was to try to grow plant in rhizotrons, which we did. Although the plants grew well and seemed quite happy, we weren't entirely satisfied with this setup for three mains reasons: we could not see all the roots, … More...

Story behind the papers

Guillaume Lobet | 2015-01-27 | open science literature

Published papers are only the tip of the research iceberg. The visible part is often a fraction of the results acquired over the years while the hidden part is made of sweat and perseverance. Of repeated cycles of failures and success. Of (lonely) hours in the lab or in front of a computer. Of writing, submitting, re-writing, re-submitting. The hidden part is where the people (we, as scientists) are. So, often, when I read a paper I wonder about the story behind the project. When did it start… More...

Plant Image Analysis Papers

Guillaume Lobet | 2012-08-12 | image analysis literature

Not all papers on root image analysis present a new software and therefore I do not present them on this website. However, some of them are worth reading since they can bring new perspectives or methods that can be useful for all root researchers. So in order to be able to share all the interesting papers I read on root image analysis, I decide to create a group on the Mendeley website (Mendeley is a free citation manager with several sharing and social features). I am therefore delighted to… More...

Using a camera to take vertical root pictures

Guillaume Lobet | 2012-06-19 | root image camera

One way to take root picture using a camera is just to hold your root system by the collar and take a picture. While this technique might be the fastest and the easiest one, it is also to easiest way to get a bad picture. Here is a couple of things to think of before taking such image. Use young plants Choose the proper camera and lens (A) Always place the camera at the same distance of the roots (B) Use a dark, matt background (C) Place a scale on the background (D) About the root system… More...

Using a scanner to take root pictures

Guillaume Lobet | 2012-06-18 | root image scanner

Using a scanner is the best way to have picture with a high contrast and resolution. However, the speed of the acquisition is rather slow compared to a camera, specially if the resolution is high. Spread your root carefully (A) Choose a sufficient resolution About the root system Scanning can be used with large root system as it is possible to separate the different root on scanner surface. It is important to carefully spread the root in order to avoid overlaps and crossing as much as… More...
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